β-lactamase

　　Penicillinase is also called β-lactamase, which hydrolyze β-lactamse. More

than 200 β-lactamase were found by far, and can be classified according to the molecular biology or the BUSH classification system, the two major classification systems for β-lactamase.

　　The molecular classification system divides the β-lactamases into four groups. Group A consists of many plasmid-encoded molecules with serine residue(s) in active site and a M.W.of 29 kDa. Group B enzymes can be encoded either by plasmids or chromosome, belong to metalloenzymes, their activity need the participation of zinc and could be inhibited by EDTA. Group C enzymes are produce in response to inducer, with active serine residue(s) an a M.W. of 39 kDa.Group C mainly refers to cephalosporinase encoded by chromosome (Amp C). Group D enzymes are also called OXA β-lactamase that hydrolyze oxacillin.

　　Another kind of classification is call BUSH classification, which also sorts the enzymes into 4 classes depending on their substrates and inhibitors spectrum. Class I includes cephalosporinase (Amp C) encoded by chromosome. Class II includes penicillinase and Extended spectrum β-lactamase (ESBL). Class III consists of metalloenzymes. Class IV contains enzymes that cannot be completely inhibited by clavulanic acid

　　Penicillinase can be used to eliminate β-lactams pollution, such as those in hospital, on medical apparatus and in foods. The enzyme we providing is TEM1, which belongs to molecular biology classification group A and BUSH classification Class II, with a M.W. of 29 kDa and PI of 5.4. We provide both natural and fusion form of this enzyme.

Physical Appearance:

Lyophilized powder

Formulation:

20mM pB, pH7.0

Solubility:

The product is soluble (1.0 mg/ml) in 20mM pB, pH7.0. The solution can be filtered through a 0.2 ì m, low protein-binding membrane.

Stability:

　　It is recommended to store the product desiccated at -18 °C . The product as supplied is stable for 1 year when stored properly. The product is stable in solution for at least three weeks at 2 - 8 °C .

Purity:

① RP-HPLC ≥ 90%

② SEC-HPLC ≥ 90%

③ reducing and non-reducing SDS-PAGE ≥ 90%

Amino-Acid Sequence :

　　The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Pro-Glu-Thr

Specific Activity:

1200 IU/mg